| abstract |
The invention discloses a livestock spermatogonial stem cell medium (SSCM). The preparation method of the livestock SSCM comprises a step of uniformly mixing 1ml of SSCB, 1mul of each of three cytokine stock solutions, 1mul of a penicillin stock solution, 1mul of a streptomycin stock solution and 1mul of a Fungizone stock solution before use. The livestock SSCM is suitable for the long-term culture of livestock SSCs. The invention simultaneously discloses a livestock SSC long-term culture method, a livestock SSC immunofluorescence dyeing identification method, a livestock SSC cryopreservation method and the like. |