abstract |
Prepare the cysteine engineered antibody that free cysteine amino acid is included in heavy chain or light chain, the i.e. nucleotide sequence of mutagenesis parental antibody and one or more amino acid residues are replaced with cysteine, so as to encoding aminothiopropionic acid engineered antibody;Expression cysteine engineered antibody;And separate cysteine engineered antibody.Some highly reactive property cysteine engineered antibodies are identified by PHESELECTOR determination methods.The cysteine engineered antibody of separation can be covalently attached to seizure mark, detection mark, drug moiety or solid support. |