| abstract |
The invention discloses a trichoderma engineering strain capable of efficiently expressing a beta-1, 4-glucanase coding gene glu14 of bacillus megaterium Ap25 source. The engineering strain L-10 is obtained by integrating fungus expression vector pSilent/glu14 containing beta-1, 4-glucanase coding gene glu14 in trichoderma viride LTR-2 chromosome with a biological control function of plant disease through a REMI (Restriction Enzyme Mediated Integration) transformation method. Beta-1, 4-glucanase coding gene glu14 can be constitutively and efficiently expressed by the engineering strain L-10, and compared with an original strain LTR-2, the growth rate and the ability of producing conidium are obviously improved. In the industrial production of the engineering strain L-10, a wettable powder is prepared by adopting liquid fermentation and a novel technology of chlamydospore induced and produced by an inducer, and adopting vector such as medical stone, so that the storage life reaches 2 years. Compared with the original strain LTR-2, a greenhouse experiment shows that the prevention effect of the engineering strain L-10 to wheat sharp eyespot and borrytis cinerea is remarkably improved. |