http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103055830-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea61bfc1125f5d765baa56a4d55c1d51 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-282 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-32 |
| filingDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf52e8f5de41712ba681c7e3bbb87eb5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c01ad4bbdc5cd68c5869e9cc21314ec4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_481469fc9f8012f2964bb525df2df983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f971fd7f83f89930e16773d08ff07f28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcd7212cbe7736136be61a3639ca7b78 |
| publicationDate | 2014-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103055830-B |
| titleOfInvention | Preparation method for solid-phase micro-extraction head based on single-stranded DNA aptamer modified graphene oxide coating |
| abstract | The invention discloses a preparation method for a novel solid-phase micro-extraction head based on a single-stranded DNA aptamer modified graphene oxide coating. The preparation method comprises the following steps: (1) subjecting a quartz fiber to silanization; (2) coating a porous polymer solution on the surface of the quartz fiber; (3) repeatedly coating a porous polymer coating; (4) carrying out silanization; (5) intercalating the silanized porous polymer coating quartz fiber into a mixed solution of a PBS buffer solution, a catalyst EDC solution, an NHS solution and an aqueous graphene oxide solution; (6) repeating step (4) and step (5) a plurality of times on the same quartz fiber; and (7) taking out the graphene oxide/porous polymer coating quartz fiber and intercalating the graphene oxide/porous polymer coating quartz fiber into a mixed solution of the PBS buffer solution, the EDC solution, the NHS solution and an aptamer solution. The preparation method provided by the invention overcomes the disadvantages of a low immobilization rate, easy shedding, instability and the like of conventional coating methods, substantially increases the immobilization rate of an aptamer and improves enrichment efficiency of SPME. |
| priorityDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 209.