http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103048470-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ae1ffe37c5b16e0b0579825b48c1ca66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2012-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1fac8ba01b5252bc07f4b67a0d38a5d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_743574655ebbb802bdce802a1776af71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6285f15c298202bc2ba1f9a200264a33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_400895367b39a45ebbb76145f5dd4bfa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8fa0de9868e25027c4a7ee21e02f4f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05dd1fd301627f6f601a411b3be64163 |
| publicationDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103048470-B |
| titleOfInvention | Immunofluorescence histochemistry technology |
| abstract | The invention belongs to the field of immunoassays and biotechnology application, and particularly discloses an immunofluorescence histochemistry technology which solves the problem that the existing immunofluorescence histochemistry technology has numerous defects when in use. The immunofluorescence histochemistry technology comprises the steps of primary dyeing: analyzing the expression of protein A and protein B in cell under test or tissue slice, then redyeing: adopting phosphate buffer to wash the cell under test or the tissue slice and performing microscopy observation till fluorescence is disappeared, sealing again: hatching corresponding primary antibodies containing protein C under test in the cell under test or the tissue slice, and then hatching secondary antibodies marked with fluorescein, and DAPI redyeing: performing microscopy through a common fluorescence microscope and taking photos, so as to obtain the microscopy photos of the protein C under test, overlying the microscopy photos with microscopy photos obtained through primary dyeing, and analyzing the expression of the protein A and the protein B and the protein C under test. The technology has high controllability, specificity and accuracy, realizes higher research requirement with lower experimental cost, and provides a wide applicable scope for the clinical immunofluorescence histochemistry. |
| priorityDate | 2012-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.