| abstract |
The invention relates to a recycling method of a methanol protein fermentation filtrate, which effectively recycles fermentation liquid during industrial production of single-cell methanol protein and comprises the steps of standing fermentation liquid obtained during production of methanol protein by a conventional method or with pichia pastoris HGD-01, settling, centrifuging, filtering, recycling the filtrate, adding ethylene diamine tetraacetic acid with the amount being sequentially increased to the filtrate during recycling for each time, mixing the filtrate with ethylene diamine tetraacetic acid to prepare mixed liquid, and mixing the mixed liquid with inorganic salt in an inorganic salt fermentation medium to prepare a substitutive medium. According to the method, the input of inorganic salt is reduced, the waste water discharge is reduced, water resources are saved, the raw material cost is lowered, the growth of yeast cells and the yield of the methanol protein are unaffected, and the production efficiency is improved. |