Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_23aa0708d5dbef5de14c266139efebf1 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 |
filingDate |
2012-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2015-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0ab373eecdbc2db088d2eff1206699c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35afb10aa3639ad615b696c4bdffa70d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dadf1fb7fd73b9b82fa8df98b62c5750 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_133dc792934235c21a7b30b944a52184 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8fcf2f535e66ac2185430afdf6a877b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14e6185fefd697d4547a071cd1772d9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8355fd1132da099d63b5698b4a3e95b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bc30db36adc20a50b9c3c2c095b79e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fae08634b1bcce7e7ca8cfea5ffa9fa2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dfece79d0c251ec1d6dd07f14544faa |
publicationDate |
2015-03-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-103045573-B |
titleOfInvention |
Cordyceps sinensis cytosine deaminase, coding gene and application thereof |
abstract |
The invention relates to an enzyme for synthesizing uracil for cytosine or for synthesizing metabolic 5-methyl cytosine from thymidine, which is participated by hirsutella sinensis of Cordyceps sinensis which is Bailing producing strain, a gene for coding the enzyme and applications thereof, wherein the enzyme is a cytosine deaminase which has more than 90% of homology with an nucleotide sequence shown in SEQ ID No.1; and the coding gene has more than 90% of homology with a nucleotide sequence shown in SEQ ID No.2. The invention detailedly researches the metabolism approach of synthesis of uracil by cytosine and synthesis of 5-methyl cytosine by thymidine in principle. The clone DNA of the nucleotide sequence provided in the invention can be transferred into engineering bacteria by a transduction, conversion and conjugal transfer method. By regulating the expression of biosynthesis genes of uracil and 5-methyl cytosine, high expressivity of uracil and 5-methyl cytosine is endowed to a host. The invention provides an effective approach for raising the output of uracil and 5-methyl cytosine, which has a great application prospect. |
priorityDate |
2012-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |