http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103038361-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91235 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9126 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07007 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
filingDate | 2011-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2017-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103038361-B |
titleOfInvention | Kit and method |
abstract | The present invention relates to a kit comprising a DNA-dependant DNA polymerase and at least one natural deoxynucleoside and to a kit comprising a DNA-dependent DNA polymerase and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being displaced from,or bound to, dsDNA synthesized by said DNA-dependent polymerase.It further relates to A method for measuring deoxynucleoside kinase activity in a sample characterized by; (i) contacting the sample, in a container, with a reaction mix comprising a DNA-dependent DNA polymerase, at least one deoxynucleoside and a detection system comprising a DNA template molecule, a DNA primer molecule, and a fluorescent moiety capable of being incorporated in, displaced from,or bound to dsDNA synthesized by said DNA dependent polymerase; (ii)incubating said container; (iii) measuring the signal from the fluorescent moiety; and correlating the signal from the fluorescent moietyto the deoxynucleoside kinase activity in the sample. |
priorityDate | 2010-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1055.