Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_23aa0708d5dbef5de14c266139efebf1 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 |
filingDate |
2012-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fae08634b1bcce7e7ca8cfea5ffa9fa2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0ab373eecdbc2db088d2eff1206699c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35afb10aa3639ad615b696c4bdffa70d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8355fd1132da099d63b5698b4a3e95b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dadf1fb7fd73b9b82fa8df98b62c5750 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14e6185fefd697d4547a071cd1772d9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8fcf2f535e66ac2185430afdf6a877b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7dfece79d0c251ec1d6dd07f14544faa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_133dc792934235c21a7b30b944a52184 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3bc30db36adc20a50b9c3c2c095b79e3 |
publicationDate |
2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-103031287-B |
titleOfInvention |
Cordyceps Chinese Hirsutella nucleoside diphosphokinase, coding gene and application thereof |
abstract |
The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic (desoxy) pyrimidine nucleoside triphosphate from (desoxy) pyrimidine nucleoside diphosphate, a gene for coding the enzyme and application thereof. The enzyme is nucleoside diphosphokinase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of (desoxy) pyrimidine nucleoside diphosphate synthesized (desoxy) pyrimidine nucleoside triphosphate in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the (desoxy) pyrimidine nucleoside triphosphate biosynthesized gene, the host (desoxy) pyrimidine nucleoside triphosphate is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the (desoxy) pyrimidine nucleoside triphosphate and having great application prospects. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104130986-A |
priorityDate |
2012-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |