http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103028145-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L31-12 |
| filingDate | 2012-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08189f100a351dccfbe3748cd8b3f45a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4836a57ab48bd523c1ce3bd5736c2e78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11a0a4530325ff7f8ccf627332c96cf7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e4b1380b80b997b6c3aca84895f840e |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103028145-B |
| titleOfInvention | Silk fibroin base integrated osteochondral two-layer bracket, preparation and application thereof |
| abstract | The invention discloses a silk fibroin base integrated osteochondral two-layer bracket, preparation and application thereof. The preparation of the silk fibroin base integrated osteochondral two-layer bracket is carried out by the following steps of: respectively soaking parts of 1/2-3/4 height of a three-dimensional silk fibroin bracket in a calcium chloride ethanol solution, absolute ethyl alcohol, a dipotassium phosphate aqueous solution and de-ionized water; repeating the above operation for 1-15 times, taking the silk fibroin bracket finally soaked by the de-ionized water to soak in the calcium chloride ethanol solution for 5-30 min and subsequently using the de-ionized water to soak the silk fibroin bracket for 3-10 min and pre-calcify the three-dimensional silk fibroin bracket; then soaking the pre-calcified part of the three-dimensional silk fibroin bracket in a bionic calcium ion buffer solution and culturing for 4-8 days at a constant temperature of 37 DEG C to obtain the silk fibroin base integrated osteochondral two-layer bracket. In the pre-calcification process of the invention, the silk fibroin bracket can obtain a nucleating locus of hydroxyapatite crystals; and evenly dispersed weak crystallization nano hydroxyapatites are formed on the surfaces of the porous channels of the bracket by culturing the bracket in the bionic calcium ion buffer solution. |
| priorityDate | 2012-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.