http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103026994-B

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grantDate 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103026994-B
titleOfInvention Factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus
abstract The invention relates to a factory hybrid breeding method of epinephelus fuscoguttatus and epinephelus lanceolatus, which comprises the following steps: selecting an epinephelus lanceolatus parent fish and an epinephelus fuscoguttatus parent fish for parent fish cultivation, artificially adjusting and controlling the cultivation water temperature of the epinephelus lanceolatus parent fish from 23 DEG C to 20 DEG and from 20 DEG to 30 DEG, controlling the cultivation water temperature of the epinephelus fuscoguttatus parent fish from 23 DEG C to 20 DEG and from 20 DEG to 28 DEG and taking mature sperm of the epinephelus lanceolatus parent fish in May-June of a next year for standby; and choosing a female fish of which an abdomen obviously expands, and an ovary develops to the stage IV, injecting a parturifacient, fishing an epinephelus fuscoguttatus female fish for artificial egg stripping after 36-hour effector phase, then mixing an collected epinephelus fuscoguttatus fish egg with the collected epinephelus lanceolatus mature sperm according to the volume ratio of 1000:1, adding sea water to finish fertilization, putting a fertilized egg into an incubation tank, collecting a floating eyed egg after about 20 hours and sterilizing by adopting ozone with a concentration of 0.3-0.5 ppm. Through the factory hybrid breeding method, the hybrid breeding of the epinephelus fuscoguttatus and the epinephelus lanceolatus is realized.
priorityDate 2013-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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