http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103014174-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d4f104f48d932d755039de2daab632e5 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
| filingDate | 2013-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f307d98f6c9125b8265897198c79b8d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d81ed2fd351eb7533e50e2bfc8ebdbe6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc88306892fcb0ffd6bd37a0b05783eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53edd58cd89045916c1d39abdc4025ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1270d69d77f7d9b5762e930eba126819 |
| publicationDate | 2015-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103014174-B |
| titleOfInvention | MPS (Macoplasmal Pneumoniae of Swine) PCR (Polymerase Chain Reaction) diagnostic kit |
| abstract | The invention discloses an MPS (Macoplasmal Pneumoniae of Swine) PCR (Polymerase Chain Reaction) diagnostic kit. The MPS PCR diagnostic kit comprises 0.1 moL/L of PBS (Phosphate Buffer Solution), a lysis buffer, DL2000, PCR enzyme, ultrapure water, primers, a positive contrast and a negative contrast, wherein the primers include an upstream primer and a downstream primer; the sequence of the upstream primer is 5'-TAGACCAGGATGGACAAGATGAT-3'; the sequence of the downstream primer is 5'-AACCACAGGACTAAGACGCAACA-3'. According to the invention, a pair of primers is designed based on the ompA gene sequence of the MPS of porcine actinobacillus pleuropneumonia in GenBank; and a PCR diagnostic kit for testing MPS is researched through optimization of PCR reaction conditions. The experimental result above shows that the MPS PCR diagnostic kit disclosed by the invention has the advantages of being fast, sensitive and accurate, and having good repeatability, long quality guarantee period and the like. |
| priorityDate | 2013-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.