http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103013886-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a3d7e466c44c7b54e2a48e4bf62665ef |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b859481de70bbbf81ee377cbe113c89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_824f1f5c133b9112109ce4da5637574e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8c6ab46ec8b5c98aaeb4215df9621ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ad934fd83e1990f2641bf451fa89606 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2feeeba080526b79eb1f2a4abdd9bd0d |
| publicationDate | 2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103013886-B |
| titleOfInvention | Preparation and regeneration method of algal toxin degrading bacterium protoplast |
| abstract | The invention relates to the technical field of water treatment microorganisms, and in particular relates to preparation and regeneration method of an algal toxin degrading bacterium protoplast. The bacterium is separated from blue-green algae dehydration filtrate and is identified to be a capitatus lysine bacillus and is preserved in the China general microbiological culture collection center, the preservation number is China general microbiological culture collection center (CGMCC) NO.6107, and the preservation name is microcystin-LR (MC-LR) degrading bacterium R3. The preparation and regeneration method mainly comprises bacterium seed culture solution preparation, hypha suspension preparation, cell wall enzymolysis, protoplast suspension preparation and protoplast regeneration. The protoplast preparation and regeneration are adopted, the preparation rate of the protoplast for degrading the capitatus lysine bacillus R3 of the MC-LR can reach 85.10%, and the regeneration rate can reach 38.40%; and moreover, the preparation and regeneration processes are low in equipment requirement and are simple to operate, the enzymolysis time and regeneration period are short, and the subsequent fusant preparation and engineering bacteria structure of R3 are benefited. |
| priorityDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 65.