http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102998444-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d927148c7982a59521f5c2ed8ae37c0 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-545 |
| filingDate | 2012-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f7799713682cf87a7596cdc9b102b1c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6d078c46ab4deea00e46ed137ab5baf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e26f3b89c7f8a98c4c76481723698e8d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e9d262610ce287fcfce19ec9866996d2 |
| publicationDate | 2014-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102998444-B |
| titleOfInvention | Stereotactic fixing method of IgG antibody on surface of polystyrene carrier |
| abstract | The invention discloses a stereotactic fixing technology of an IgG antibody, wherein the IgG antibody fixed on the surface of a polystyrene carrier is uniform, the Fab segment is exposed adequately and the high antigen binding activity of the IgG antibody is maintained, so that the sensitivity, specificity and stability of solid phase immunoassay are effectively improved. The stereotactic fixing method of IgG antibody provided by the invention specifically comprises the following steps: grafting a 'metal ion arm' on the surface of polystyrene by a chemical modification method; constructing a ZZ affinity peptide with Histag by a genetic engineering technology; mediating the ZZ affinity peptide based on Histag and metal ion affinity characteristics to be stereotactically fixed on the surface of polystyrene, so as to exert the immunological properties of the ZZ affinity peptide; specifically combining the Fc section of the IgG antibody to obtain a metal ion-(ZZ-His)-Fc assembled body in a biological affinity mode, so as to stereotactically fix the Fab segment of the IgG antibody. |
| priorityDate | 2012-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.