http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102994652-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cfaed36e9f699b0a1846b5b4fd0af98e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_598ebdc529b456c7e98aa57530cc2fc3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3eb53b5582cab73a18dd6e76fcbdb98a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44ecea1af98df9e879943e09ebe7f113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e55f371ea95892160a46b4b72c4d925 |
| publicationDate | 2015-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102994652-B |
| titleOfInvention | Method for detecting newcastle disease virus nucleic acid by RT-PCR |
| abstract | The invention relates to a virus detection method, and particularly relates to a method for detecting newcastle disease virus nucleic acid by RT-PCR, which comprises virus multiplication, primer design, nucleic acid extraction, product identification, and sequence identification; the beneficial effects of the invention are that: according to the method, virus multiplication is performed firstly by chick embryo inoculation, and then PCR detection is performed; an R degenerate primer is added into an F-F primer, which realizes the detection of week viruses and strong viruses during the detection of the newcastle disease virus nucleic acid; the detection is accurate, reduces the time for respectively detecting week viruses and strong viruses, and solves the problem of easy detection missing in traditional methods. |
| priorityDate | 2012-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.