http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102994550-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866 |
| filingDate | 2012-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102994550-B |
| titleOfInvention | The method of expression alien gene in zooblast or animal tissues |
| abstract | The invention discloses a kind of method of expression alien gene in zooblast or animal tissues.The invention provides a kind of Bombyx mori nuclear polyhedrosis virus foreign gene being imported mammalian cell or carry out gene delivery in organizing, comprise skeleton carrier silkworm baculovirus BmNPV and the expression cassette of the foreign gene on skeleton carrier of recombinating; The expression cassette of described foreign gene is made up of mammalian promoter, foreign gene and terminator.The present invention further discloses a kind of method of expression alien gene in zooblast or animal tissues, comprising: by the Bombyx mori nuclear polyhedrosis virus infected insect host of structure or insect cell; Cultivate infected insect host or insect cell increases to Bombyx mori nuclear polyhedrosis virus; Results are purifying institute amplified production also.Silkworm baculovirus gene delivery carrier constructed by the present invention have in zooblast can not increase, can not carry out with zooblast genome recombinating, external a large amount of amplification is convenient, security advantages of higher. |
| priorityDate | 2012-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 136.