http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102986648-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d63f2f172458d22bac1e0aff61bc1372 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2012-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00962051fe59e6c8043e7142ad5088a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_719053db67139d64152a362f1f9d643f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70da5a4530052bb3b1c37cb84b149065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b81493f4b31934f61a087ed05a9251a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbb33d693055da28283972693590bbce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e592d28e509f76afc2c6c8018c08ec8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35944990da42398a4d2388aa38266f2e |
| publicationDate | 2014-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102986648-B |
| titleOfInvention | Vitrified cryopreservation and thawing recovery method for bursaphelenchus xylophilus |
| abstract | The invention discloses a vitrified cryopreservation and thawing recovery method for bursaphelenchus xylophilus. The method comprises the following steps of: (1) mixing bursaphelenchus xylophilus suspension and a cryoprotectant uniformly to obtain a mixture, and balancing the mixture; (2) performing cryopreservation on the balanced mixture in liquid nitrogen; and (3) thawing and recovering, wherein the cryoprotectant consists of dimethyl sulfoxide and yolk. Each process parameter in the vitrified cryopreservation method is optimized, and the category and the concentration of the cryoprotectant optimal for the vitrified cryopreservation of the bursaphelenchus xylophilus and an optimal treatment mode comprising pretreatment, balancing and thawing recovery are screened. By adopting the method for cryopreservation of the bursaphelenchus xylophilus, the frozen bursaphelenchus xylophilus is high in survival rate and reproductive rate; and the method can be applied to cryopreservation of different strains of the bursaphelenchus xylophilus. |
| priorityDate | 2012-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 68.