http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102977160-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H15-203 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52 |
| filingDate | 2012-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102977160-B |
| titleOfInvention | A kind of 4-nitro-1-naphthols or derivatives thereof is method and the application thereof that beta-galactosidase enzymes chromogenic substrate is prepared by colour developing group |
| abstract | A kind of 4-nitro-1-naphthols or derivatives thereof is method and the application thereof that beta-galactosidase enzymes chromogenic substrate is prepared by colour developing group; First by all hydroxyls of β-D-galactopyranose with including but not limited to that the short chain carboxy acid of formic acid and acetic acid generates ester, alpha-brominated semi-lactosi polycarboxylate is reacted to obtain again with the formic acid of HBr or acetic acid solution, react to obtain glucosides ester in acetone with 4-nitro-1-naphthols or derivatives thereof in the basic conditions further, the β-D-galactopyranoside that deacylation obtains 4-nitro-1-naphthyl-β-D-galactopyranoside or 4-nitro-1-naphthol derivative in methyl alcohol or ethanol is chromogenic substrate; This type of chromogenic substrate is color product through beta-galactosidase enzymes effect release 4-nitro-1-naphthols or derivatives thereof, is suitable in wider pH range, measure color product and absorbs tracking reaction process determination beta-D-galactosidase activity. |
| priorityDate | 2012-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 188.