http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102965329-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5e6dd1ed680b7a8f7f9171177ee2b48a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-04 |
| filingDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df992645402a6bacc1d751cbd2de64d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8efe57c99662c7e7e9c8161118a5651 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d03eca1b68e7f63f453290968c669cf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5981adf21059958a05ea8f4742534578 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f94738b3e8f01018c8ed6597ce833a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e35b037977367182effa9534b220bb9f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_66bd13295780d96275982e6bfe895104 |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102965329-B |
| titleOfInvention | Method for stably culturing taxus chinensis plant cell seeds |
| abstract | The invention discloses a method for stably culturing taxus chinensis plant cell seeds, and aims to provide a method which is capable of being used for stably culturing the taxus chinensis plant cell seeds; and for the method, the advantages of being economically feasible, simple and controllable in process, and low in cost are realized. The method comprises the steps of: (1) sampling and detecting subculturing seeds; (2) adjusting a final biomass of the seeds to 5-10g/L; (3) increasing the initial sugar degree of a culture solution to 15-25g/L; (4) increasing the initial electric conductivity of the culture solution to 2.5-3.5ms/cm; (5) controlling an initial biomass of the culture solution to be in the range of 2.5-3.5g/L and the initial pH (potential of Hydrogen) to be in the range of 5.0-6.0, stirring and carrying out seed subculturing; and (6) when the subcultured seeds in the step (5) meet the requirements that A, the final sugar degree of the culture solution is greater than 8g/L or B, the final electric conductivity of the culture solution is greater than 2.0ms/cm or C, the final pH of the culture solution is less than 6.0, starting next seed subculturing. The method belongs to the technical field of seed culturing. |
| priorityDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 53.