http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102964443-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2012-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102964443-B |
| titleOfInvention | Construction and production methods of recombinant vector of recombinant chicken gamma interferon |
| abstract | The invention relates to techniques of gene clone, recombinant vector construction, recombinant plasmid expression and purification and the like in the technical field of genetic engineering, and particularly relates to construction and production methods of recombinant vector of recombinant chicken gamma interferon. The construction method mainly comprises the following steps of: acquiring target genes containing complete open reading frames of the chicken gamma interferon or segments desired to be improved by an RT-PCR (reverse transcription-polymerase chain reaction) technique, constructing expression vectors containing the target genes, and meanwhile, constructing the processes of inducible expression and separation and purification of target proteins. According to the construction and production methods of the recombinant vector of the recombinant chicken gamma interferon, the chicken gamma interferon is enabled to be efficiently expressed in escherichia coli, a fast, high-efficient, safe and low-cost production manner is provided for the production of the chicken gamma interferon, and the construction and production methods of the recombinant vector of the recombinant chicken gamma interferon are suitable for large-scale production. The recombinant chicken gamma interferon produced according to the invention can be widely applied to immunological enhancement of poultries and prevention and cure of plague. |
| priorityDate | 2012-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 78.