http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102952874-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f3d4964b5a4fef7e756d169c4a0b9c75 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2011-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a50592c9cf477822419f04b8c7956949 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27cd769b6c43f11d1b233c1f38a7656b |
| publicationDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102952874-B |
| titleOfInvention | Specific primer and liquid chip for detecting polymorphism of SLC22A1 gene |
| abstract | The invention discloses a liquid chip and specific primer for detecting an SLC22A1 gene. The liquid chip mainly comprises ASPE (allele specific primer extension) primers, microspheres and amplification primers, wherein each ASPE primer is formed by tag sequences at the 5' terminal and specific primer sequences which are arranged at the 3' terminal and aim at the mutation site of the target gene; the specific primer sequences are SEQ ID NO.19 and SEQ ID NO.20 aiming at the C117T site, SEQ ID NO.21 and SEQ ID NO.22 aiming at the T198C site, SEQ ID NO.23 and SEQ ID NO.24 aiming at the C86T site, SEQ ID NO.25 and SEQ ID NO.26 aiming at the C97G site, SEQ ID NO.27 and SEQ ID NO.28 aiming at the C164T site, SEQ ID NO.29 and SEQ ID NO.30 aiming at the G123T site, SEQ ID NO.31 and SEQ ID NO.32 aiming at the G127A site, SEQ ID NO.33 and SEQ ID NO.34 aiming at the A148G site and/or SEQ ID NO.35 and SEQ ID NO.36 aiming at the G129C site; and the microspheres are enveloped by anti-tag sequences. The detecting liquid chip provided by the invention has the advantages that the consistency of the detection result and the sequencing method is as high as 100%, thus parallel detection of wild types and mutant types of a plurality of mutation sites is achieved. |
| priorityDate | 2011-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 24.