http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102952776-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24324b37f9f894773efbcfcced4e4138 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2012-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d8855b06f898bdb1f2bf1d765a577df http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99110833287f086948e7f4a4b5f57fe1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85d5b8c03b7f7d13c4c0a4fc09ac68f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d38f5b510891ee74e8567ad001a46610 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19c190903d84e4c2cbf542f4d4133d8b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eefbd8a25d5a50d9999aaa2df6e62cd7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a38b959515e0e7f2e021837dab032a1a |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102952776-B |
| titleOfInvention | Culture method for eriones unguiculatus primary liver cells |
| abstract | The present invention discloses a culture method for eriones unguiculatus primary liver cells. The method comprises: (1) collecting a liver cell primary suspension from digested and matured isolated eriones unguiculatus liver, filtering, removing impurities from a filtrate, adding 5% calf serum-containing HBSS, repeatedly carrying out centrifugation washing, collecting cell precipitate and adding to a 20% new born calf serum-containing DMEM complete culture medium, and adjusting a liver cell concentration to 3.0*10<5>-5.0*10<5>/mL to prepare a liver cell suspension; and (2) inoculating the liver cell suspension in a culture plate coated with rat tail collagen, culturing at a temperature of 37 DEG C in a 5% CO2 environment, removing impurities after 4 hours, changing the culture medium into a maintenance culture medium containing cell factors, and carrying out cell adherence overnight to obtain the eriones unguiculatus primary liver cells. The method has characteristics of low cost, economy, practicality, and simple and easy-performing operation, wherein the obtained liver cells have characteristics of strong adherence, high vitality, high survival rate, stability and reliability, and the method is suitable for mass production. |
| priorityDate | 2012-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.