abstract |
Provide dimer and trimeric nucleic acid dyes and relevant system and method.This dyestuff can form hair clip spline structure, and this structure makes described dyestuff make nucleic acid painted by (such as) on-demand releasing mechanism.Such as, this dyestuff is not having the background fluorescence under nucleic acid low, and under having nucleic acid to exist, the fluorescence after being combined with nucleic acid is high.Dyestuff provided by the invention can be used for various field, such as, DNA quantitative assay in PCR in real time. |