http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102936619-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2014-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102936619-B |
| titleOfInvention | Method for quantitative determination of escherichia coli RNA, and specialized standard substance and application thereof |
| abstract | The invention discloses a method for quantitative determination of escherichia coli RNA, and a specialized standard substance and an application thereof. The method comprises the steps of (1) making a standard curve according to the following method, preparing standard substance diluents with different concentrations by using a RNA standard substance; reversely transcribing the standard substance diluents with different concentrations into cDNA; performing real-time fluorescence quantification PCR by using cDNA as templates; making the standard curve equation by using RNA copy number (or data-processed copy number, for example, log-base 10 of the copy number) corresponding to cDNA in the real-time fluorescence quantification PCR system and Ct values; and (2) extracting a total RNA of the escherichia coli, reversely transcribing the extracted total RNA into cDNA; performing the real-time fluorescence quantification PCR by using cDNA as the templates; and substituting the Ct values to the standard curve equation to obtain the RNA content of the escherichia coli. The method can be used for detecting small amount of the escherichia coli in the environment rapidly, and provides technical support for rapid detection of the active escherichia coli. |
| priorityDate | 2012-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.