| abstract |
The present invention relates to a group of anti-human CD146 molecules developed by using a biological technology and an established high sensitivity sandwich ELISA method for soluble CD146 detection. The present invention provides anti-human CD146 mouse monoclonal antibodies (AA1, AA2, AA3, AA4, AA5 and AA7), and a method for specially detecting soluble CD146 by using antibody combinations and sandwich ELISA, wherein the antibodies can specifically recognize human source CD146 at a molecular level, a cellular level and a tissue level, and can be divided into two classes based on recognition of different epitopes. With an antibody AA1 and anti-human CD146 mouse monoclonal antibody AA98 combined sandwich ELISA method, nanogram-scale soluble CD146 in per ml of the solution can be detected. The antibodies and the detection method can become effective detection or diagnosis tools and methods in basic researches or clinical applications, and can further provide good carriers for targeted therapies of CD146-related diseases. |