http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102925559-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2effa7afdc95cf341ad587762458727d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6d3821212cd8a2790cf75d7ff329d51a |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df5bad0ca2977ce3c2a314147ac7fea6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7664f279690682339ddc3748c4ee8e8 |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102925559-B |
| titleOfInvention | Kit for quantitatively detecting W515 site mutation of MPL genes |
| abstract | The invention relates to a kit for quantitatively detecting the W515 site mutation of MPL genes, belonging to the field of biotechnology. The kit comprises at least one system of the W515L site mutation specific system of the MPL genes and the W515K site mutation specific system of the MPL genes, and an inner reference gene ABL system, wherein each system comprises an upstream primer, a downstream primer and a Taqman fluorescent probe. Research shows that the gain-of-function mutation rates of the W515L site mutation of the MPL genes and the W515K site mutation of the MPL genes are 10% and 3% in PMF and ET respectively, and are not found in PV. Therefore, the W515L site mutation of the MPL genes and the W515K site mutation of the MPL genes are mainly used for diagnosing the PMF and the ET. With the adoption of the fluorescent quantitative PCR (polymerase chain reaction) which is high in sensitivity and specificity for detecting the W515L site mutation of the MPL genes and the W515K site mutation of the MPL genes, the specificity and the sensitivity of the detection result are remarkably improved. Via the kit disclosed by the invention, a novel rapid, simple and convenient gene diagnosis technology is provided for prediction for myeloproliferative diseases. |
| priorityDate | 2012-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.