http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102919132-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd9b7850804b363c1e7aa9ee33e97b17 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2012-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af8af27b8bb495c06f3f392062e71f84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc20eaf6eb55e36be374126ca3197864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8371296acc2d3db8b0f50b5e2685479b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2a32745244c903f3d47c9a5d676d92f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6458f1559b07ab81ee2e16f03926644f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d3c630ad8d54188c4ed053764023917 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cf2c4212f1ed8b7169dbb1c128e20db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61c28aad7f59a3ec0374998e9c76e95d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b766315392f1961a72ddafe83aac95ed |
| publicationDate | 2013-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102919132-B |
| titleOfInvention | Tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine |
| abstract | The invention relates to the technology of plant tissue culture, and in particular relates to a tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine. The method taking petiole of populus yunnanensis dode as a tissue culture material in the process of tissue culture is characterized by comprising the following steps of: adding colchicine into a populus yunnanensis dode petiole differential medium to induce populus yunnanensis dode to generate polyploidy, wherein the treatment time is 20-30 days; carrying out differentiation culture again in a populus yunnanensis dode petiole differential medium in which colchicine is not added after the treatment; cutting the part which is obvious in variation on the differentiated cluster buds; repeatedly cultivating on the differential medium for 5-6 times, so that the populus yunnanensis dode petiole differential medium is stable in polyploidization variation; and performing rooting on the clump seedlings which are stably varied, so that the tissue culture seedlings of the tetraploid populus yunnanensis dode can be obtained. The method provided by the invention is the tissue culture method for inducing the populus yunnanensis dode tetraploid in combination with the colchicine, which is easy and simple to operate and can effectively reduce the chimera. |
| priorityDate | 2012-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.