http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102911958-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4fb38ebd40c70e97cc5f1519c6699162 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2012-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5e46baac6e24a1c24fb58ab7a06d505 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f8047215d09807abe34089a809e1322 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d27240733d60035a338384b90b6db449 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e9236229fb0c5954b869169d257c6d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9252c2d5c7aca4c3982875e4dbd4d5c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ca512e969d472f960e7e5854ba1a5c7 |
| publicationDate | 2014-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102911958-B |
| titleOfInvention | Gene for coding recombinant human TNFR-Fc fusion protein and application of gene |
| abstract | The invention discloses a gene for coding a recombinant human TNFR-Fc fusion protein and an application of the gene. The gene for coding the recombinant human TNFR-Fc fusion protein as shown in SEQ ID NO.1 is obtained by optimized screening of codons. The gene has high expression index in a CHO (Chinese Hamster Ovary) cell, and the expressed protein has high affinity with TNFR. The gene is transferred into the CHO cell to obtain a cell for expressing the recombinant human TNFR-Fc fusion protein. The CHO cell into which the gene is transferred is fermented by using a disposable reactor, the operation is simple, and the obtained protein quantity is higher in comparison with the conventional fermentation tank; and especially after a supplemented medium is added, the cell growth time can be prolonged, the expression level can be increased, the production cost can be reduced, and a high-purity target protein can be obtained. |
| priorityDate | 2012-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 279.