http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102899408-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_86eccabdd1b630e10ce6f7781fd85283 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2012-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4372f1ad7794f77f7a57838faf74a70f |
publicationDate | 2014-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102899408-B |
titleOfInvention | Sequencing primer for qualitative detection of KRAS genetic typing and kit thereof |
abstract | The invention provides a sequencing primer for detecting mutation of codons 12 and 13 in a second exon and a codon 61 in a third exon of a KRAS gene, and a kit thereof, and belongs to the field of in vitro nucleic acid detection. The kit comprises uracil DNA glycosylase, Taq polymerase, PCR amplification primers and sequencing primers. The kit provided by the invention has high sensitivity and good specificity; the PCR products can be simply treated for sequencing on a phosphate sequenator; and the kit has advantages of simple operation, short reaction time, and higher sensitivity than a gold standard-capillary electrophoresis sequencing, and is more suitable for mutation analysis. |
priorityDate | 2012-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 273.