http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102899347-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f90c1df3857ee72900e0015c75caecd9 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
| filingDate | 2012-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_878a65d439992a7b82bc61664fdc227e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b7b08f9c08c047d579707080cebcc825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa029013ce881e3c7b1fc8eae2f832e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0148505354b990369354cce4cc4cc914 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a65fc8c50036baa93cfb88d3ad0aba4b |
| publicationDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102899347-B |
| titleOfInvention | Method for constructing high-yield chitinase bacillus thuringiensis through gene knockout |
| abstract | The invention discloses a method for constructing high-yield chitinase bacillus thuringiensis through gene knockout, belonging to the technical field of bioengineering. The method comprises the steps of: firstly, with bacillus thuringiensis ATCC35646 (American Type Culture Collection) as an original strain and a temperature sensitive carrier pKSV7 as a framework, constructing a gene knockout carrier pKSV-ued; and then based on a homologous recombination principle, through a method for knocking a yvoA gene out of a strain genome of the bacillus thuringiensis ATCC35646 through a double exchange process, constructing a high-yield chitinase strain CJ212. Through enzymatic determination of chitinase, the capacity of producing the chitinase by the improved strain is greatly improved. |
| priorityDate | 2012-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.