| abstract |
The invention relates to a duck hemorrhagic ovaritis virus (DHOV). Tissue samples of clinical diseased egg ducks are finely separated by SPF (specific pathogen free) chicken embryo fibroblasts (CEF) to obtain a DHOV-JN, and the isolate can proliferate on CEF and produce a typical cytopathoenic effect (CPE), and can also proliferate one MDCKMadin-Darby canine kidney, Vero, BHK-21(baby hamster kidney-21), ST (Swine Testis) and Marc-145 cell monolayer and produce more consistent CPE, and the tilter of the isolate is 105.5TCID5(Tissue Culture Infectious Dose)50/0.1mL; the isolate belongs to a member of flaviviridae, yellow disease and Ntaya virus group; and the strain can develop corresponding RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection/diagnosis methods (reagents) based on the study, and can also develop vaccines and other biological products for clinical prevention of the disease. |