http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102876642-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_39f81efde7c8193eb9baec2c78443536 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d94573ab2c57e8b193f3a22accddf9fe |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-18 |
| filingDate | 2012-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f987c89fa2ce7489ec352e6eac341f16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_900398208f8f36a811cbaa3e7cc7c8b0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a72f5a3e0d9e3a223f3d02774e385530 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a8ff26b099a401c5b52ec9db0a2433e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_558ce7ab935d8b6748cbb9b3184b3799 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1f9d2f33a77a32d2cb91bdb11f1a996 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b5d5f29cf4a9e243390e731a72c6817 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37ba617a7c3c1249dd31f31032d814d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_34afaca4ac116f82bb9f13896705914f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c456668c58122fbef1e1c5c3ccd8c9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cf38b5e939e1cf4229b9cba95473020 |
| publicationDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102876642-B |
| titleOfInvention | Preparation method for pyrethroids pesticide degrading enzyme |
| abstract | The invention relates to the technical field of a preparation method for pesticide degrading enzyme, in particular to a preparation method for pyrethroids pesticide degrading enzyme. The preparation method for the pyrethroids pesticide degrading enzyme comprises the following steps of: 1, taking recombinant escherichia coli (E.coliBL21(DE3)/pET-28a-est825) as a production strain, and storing the production strain at the temperature of 86DEG C below zero by a glycerol tube; 2, carrying out seed culture; 3, carrying out fermentation culture; 4, carrying out feed supplement and fermentation culture; 5, carrying out lactose induction; and 6, preparing enzyme powder. According to the preparation method for the pyrethroids pesticide degrading enzyme, the recombinant escherichia coli is adopted to efficiently express the pyrethroids pesticide degrading enzyme, the yield of the finally obtained degrading enzyme powder is 25-40g/, the enzyme activity of the enzyme powder is 48000-60000U/g, the yield of the pyrethroids pesticide degrading enzyme is greatly improved, the use cost of the pyrethroids pesticide degrading enzyme is lowered, and an effective, economic and feasible path is provided for the mass production of the pyrethroids pesticide degrading enzyme. |
| priorityDate | 2012-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.