http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102866220-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2832a40f112f92bf8d7655e3809f5815 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 |
| filingDate | 2011-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b75623f3f4839590b61cd1445c7c33b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_18455947bbb6e03f940bab2f0e5e2921 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b5a959c7b2e29c554d4ee9e5244d9c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b1e55058b446f78e05c50c7f956edf1 |
| publicationDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102866220-B |
| titleOfInvention | Chemical derivation-based detection method for hemolymph metabolin of migratory locust |
| abstract | The invention discloses a chemical derivation-based detection method for hemolymph metabolin of migratory locust, and relates to the field of animal ecology and analytical chemistry. The detection method specifically comprises the following steps of: taking hemolymph, deproteinizing by adding ethanol-acetonitrile mixed solvent, supersonically extracting, and centrifuging at a high speed to obtain metabolin extracting solution; drying the extracting solution by means of pressure reduction, adding methoxamine hydrochloride-pyridine solution, supersonically dissolving, blending by means of vortex, and deriving in an oximation way in a constant-temperature water bath; adding N-methyl-N-trimethylsilane-trifluoroacetamide reagent, blending by means of vortex, and deriving in an alkylation way in the constant-temperature water bath; and adding methyl oleate-normal heptane solution to compensate the volume, blending by means of vortex, and analyzing by a spare gas chromatography-mass spectra coupling technique. The detection method provided by the invention is convenient, high-efficiency, mild in reaction conditions, good in repeatability, wide in metabolin coverage, suitable for developing the multi-center and large-sample research, and capable of providing help for knowing the biological law of various migratory locust variations and the occurrence mechanism of the locust plague. |
| priorityDate | 2011-07-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 66.