http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102864172-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b93c2aafeb1bcb1019daee345cc8f141 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
| filingDate | 2012-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44cc58918cbf97a624984e78d8216e8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de7d6fa56c03ae17e90e1c2432da1b64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6af0357821fb188bdef84b7e73c35bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_980b824719281b6ee2616841c12d8b54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f757deb8544acb0c87ec1979bcdb143c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10b5f18e94b6e7a01e3e1755e69b048e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4352a7457a6fc6e12010f6e4659c6795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e89c0e5252d3fc6a913704463cb2743 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_379235da9deee632f64e9b9ecc0e91cb |
| publicationDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102864172-B |
| titleOfInvention | Leukemia mouse model based on gene co-transfection technology and preparation method thereof |
| abstract | The invention relates to the technical field of biology, in particular to a leukemia mouse model based on a gene co-transfection technology and a preparation method thereof. The preparation method of the leukemia mouse model comprises the following steps: performing construction and packaging of K-ras mutants and AML1-ETO fusion gene lentivirus vectors; performing bone marrow cell separation and virus infection condition monitoring; implanting infection cells in a mouse to build a leukemia animal model; and performing model identification. The method of building the leukemia mouse model in a mode of utilizing caudal vein injection to lead in the manual site-directed mutagenesis K-ras mutants and AML1-ETO fusion gene lentivirus vectors is adopted initiatively. The leukemia mouse model is high in success rate, pathological characteristics are high in similarity to morbidity conditions of clinical leukemia, and the novel animal model can be provided for leukemia extramedullary infiltration mechanism research, leukemia medicine screening and gene and molecule target treatment. |
| priorityDate | 2012-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.