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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
filingDate 2011-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2015-08-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102844433-B
titleOfInvention The detection method of PCR primer pair, PCR reaction solution and food poisoning bacteria
abstract A kind of PCR primer pair, the nucleic acid of the two or more food poisoning bacteria in its specific amplification bacillus cereus, Campylobacter spp, intestinal bacteria, listeria bacteria, Salmonellas, streptococcus aureus, Vibrio parahaemolyticus.Thus, can simultaneously and detect these food poisoning bacterias specifically.Use the PCR primer pair be made up of the plural primer pair among following primer pair, utilize PCR method, carry out specific amplification to the nucleic acid of food poisoning bacteria, described primer pair comprises: for the sequence numbering 1 and 2 of bacillus cereus detection, the primer pair of sequence numbering 3 and 4; For the primer pair of the sequence numbering 5 and 6 that Campylobacter spp detects; For the primer of the sequence numbering 7 and 8 of E. coli detection; For the primer pair of sequence numbering 9 and 10, the primer pair for the sequence numbering 11 and 12 of Salmeterol fluticasone propionate that listeria bacteria is detected; For the primer pair of the sequence numbering 13 and 14 that streptococcus aureus detects; For the primer pair of the sequence numbering 15 and 16 that Vibrio parahaemolyticus detects.
priorityDate 2010-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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