http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102839185-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f90c1df3857ee72900e0015c75caecd9 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P3-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-02 |
| filingDate | 2011-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea44b403a391a7efde1e50af3213ea2c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed6c5aa98a1f0203d11f3264321e10c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de88d62d7ee9479df0bba4e50a41b5af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6315b3429de67acf64e74a4e7af66fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9cca7cbd7f1ffd7eb12e9b0d2afb343 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31d8289e4dd443aa819cb9d45f1eb5d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b0876d610ec89957ea62b8b613ab13d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc591ed6e0875dc7e7362dc7984f78ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_749d662bf15ceccaabeb8935af4705de |
| publicationDate | 2014-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102839185-B |
| titleOfInvention | Construction method and application technology of rolGLP-HV engineering bacterium |
| abstract | The invention relates to a construction and preparation method and an application technology for producing a dual-functional medicine (rolGLP-HV) engineering bacterium for treating diabetes and thromboembolic complications. The construction method disclosed herein is characterized by fusing long-acting insulinotropin rolGLP-1 having the effect of treating the symptoms and causes of type 2 diabetes with hirudin rHV1 which can effectively control cardio-cerebrovascular complications to achieve the dual functions of preventing and treating diabetes and thromboembolic complications. Specifically, by applying gene engineering technique, the fused gene rolGLP-HV of rolGLP-1 and rHV1 is obtained; by cloning the fused gene rolGLP-HV to an escherichia coli expression vector pET22b(+), an expression vector pNK-EC-rolGLP-HV is constructed; by conversing escherichia coli, the engineering bacteria NK-rolGLP-HV-E007 with highly expressing rolGLP-HV is obtained; and by conducting inducible expression and purification of the strain, the high-purity rolGLP-HV can be obtained. According to the invention, by perfusing the rolGLP-HV into the stomach of the Hu thrombus model mice, the thrombosis can be significantly slowered and alleviated; by perfusing the rolGLP-HV into the stomach of the diabete model mice, the blood glucose can be obviously reduced, and diabete symptoms can be improved, thus the rolGLP-HV disclosed herein can be used for developing the dual-function medicines for simultaneously treating diabetes and thromboembolic complications thereof. |
| priorityDate | 2011-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 145.