| abstract |
The invention discloses enterobacter capable of producing esterase, a protein sequence of the esterase produced by the enterobacter, an gene of the esterase, a recombination expressor containing the gene, and a recombination express transformant. The invention also discloses an application of utilizing the whole enterobacter cell to prepare and produce chiral taxol precursor (2S, 3R)-phenyl glycidic acid methyl oleatethrough enantioselective hydrolysis of the esterase or the recombinant esterase catalyzing racemization (+-)-phenyl glycidic acid methyl oleate. The enterobacter (Enterobacter sp.) ECU 1107 is stored with a number of CGMCC No. 5981. With adoption of the whole enterobacter cell, the esterase or the recombinant esterase is adopted to catalyze to prepare the taxol precursor; a technology is simple, a reaction condition is mid, catalyzing efficiency is high, and a product has good optical purity; and 99% of enantiomer excess (ee) can be achieved; and a good industrial application prospect is provided. |