http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102816841-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6474bcaf9f7b4f98e4f9643e3f3cd606 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-63 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75a8bb3dc0ea5fc39c6606e17997c394 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a2e7c095c6a8e200e3ec121ed4495ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc0e47128b453de2cb0b8e60ba90c907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ff353a5bb3c3fa0fdf3459050450be4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64394756dcef0a270a88f5a3ac1ae2c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96ad2354701ddb8d8e727af90af61a48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a06c9ee0e146c91d82ea1fb13a8fc96f |
| publicationDate | 2014-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102816841-B |
| titleOfInvention | Four-color fluorescent PCR kit for detection of vibrios in water body and detection method |
| abstract | The invention discloses a four-color fluorescent PCR kit for detection of vibrios in a water body and a detection method. According to the method, innovative bacterium enrichment reagent technology, DNA extraction reagent technology, specific fluorescent probe hybridization PCR detection technology and multiplex PCR technology are employed, bacterium enrichment culture of a sample is not needed, false positive interference is minimized as much as possible on the premise that high sensitivity is maintained, and four populations of two kinds of vibrios can be detected at one time. The invention mainly has the following beneficial effects: no need for bacterium enrichment of the sample in advance; high sensitivity and good specificity in detection of bacteria; reduction of the false positive incidence in conventional PCR amplification; and realization of rapid, accurate and specific detection of vibrio cholera and vibrio parahaemolyticus at one time. |
| priorityDate | 2012-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.