http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102816711-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5cedae1e4103c4e3719a5e9c94c2b510 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2012-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2cae0c2a8822ec921c20177592a1d662 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a33102ab573b35014c73209297a74af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b5b0eca06604576d18b7b42342ee967 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ac0470a9e0b3b0ebb72c251adf96353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b73ec476825f996926d114dc8d19d9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c8bfc1121d8a376a8db01791e47aabf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c80f014d46d1f3bf89535850186e43c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85a8ebdfc19a3cd3c0757495e464f3e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f760c67e1ee7d551288644dbf397848e |
| publicationDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102816711-B |
| titleOfInvention | Preparation method and conversion method of bifidobacterium protoplast |
| abstract | The invention belongs to the microbial field, and provides a preparation method and a conversion method of a bifidobacterium protoplast. The preparation method comprises the following steps: inoculating Bifidobacterium longum into an MRS argar culture medium, carrying out anaerobic culture, sub-culturing into an MRS liquid culture medium, culturing, sub-inoculating 2.5-3.5% of the substance cultured in the MRS liquid culture medium into a fresh MRS liquid culture medium, and carrying out anaerobic culture at 36-38DEG C for 18-20h; collecting thalli, and carrying out heavy suspension; adding mutanolysin to the final concentration of 4.5-5.5mg/L, and carrying out enzymatic hydrolysis at 36-38DEG C for 25-35min; and carrying out heavy suspension in an SMM solution to obtain the bifidobacterium protoplast. The generation rate of the protoplast prepared through the method reaches 81%, the regeneration rate of the protoplast reaches 48, and the protoplast can be applied to the conversion of bifidobacteria through exogenous gene introduction to construct engineered strains of the bifidobacteria. |
| priorityDate | 2012-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 110.