http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102816401-B

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filingDate 2012-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0d2155bf8e896f014cb5dc540c49966
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publicationDate 2013-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102816401-B
titleOfInvention Embedding medium suitable for plant tissue frozen section and frozen slicing method
abstract Relating to methods able to conduct fast histochemistry and other analyses in plant tissues, the invention specifically relates to an embedding medium and a direct frozen section method. The embedding medium is composed of polyvinyl alcohol, polyethylene glycol, carboxymethylcellulose sodium, glycerol, formaldehyde, collagen, plant gel, and deionized water. According to the frozen section method, a plant material is not fixed, and a sample subjected to histochemical staining and other treatments or a fresh tissue is wrapped on a sample table directly to perform frozen section. The method solves the problem that in the microscopic observation of phytohistochemistry, immunolocalization, in-situ hybridization and other studies, a target chemical substance is easy to dissolve in a fixation agent, and is impossible to be fixed first and then sectioned, or the target chemical substance dissolves in an alcohol substance and is impossible to be dehydrated by ethanol.
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