http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102796812-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_374e1088d4ea8f45a9b5396d4302ec03 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_966249357bcffcdb2151867c1c4a1643 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdd4b14e7a94c860ada84cc72d741e7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8124e1f3b59819dadebbf5278f5b2c97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af403ecfb5143b71bf140cbda7346b83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0602e04df4c524db0713bc59ca8c5ece http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc817590c2a9ad86521d254559d5f97e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13d41c45bda0ca12ab22dc85817a1909 |
| publicationDate | 2013-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102796812-B |
| titleOfInvention | PCR (Polymerase chain reaction) primer capable of simultaneously detecting five soil-borne fungal diseases of wheat paddock and detecting method thereof |
| abstract | The invention discloses a PCR (polymerase chain reaction) primer capable of simultaneously detecting five soil-borne fungal diseases of wheat paddock, wherein the sequence of the rhizoctonia solani primer is as follows: an upstream primer P1: 5'-TCCTCCCGCTTATTGATATGC-3'; and a downstream primer P2: 5'-CGGTTGAGCTGGGTCTTTTA-3'. A multi-PCR detecting system built by the invention can simultaneously detect the five pathogenic funguses such as rhizoctonia cerealis, gaeumannomyces graminis, fusarium graminearum, fake fusarium graminearum and bipolaris sorokinianum. The size of an amplified product segment disclosed by the invention is accordant with an expected result, and five pairs of the primers only can be generated aiming at the target specific stripe and the non-specific stripe, so that the five pathogenic funguses can be well distinguished from one another. The PCR primer is convenient and quick: a sample can be prepared and detected within four hours, and the five pathogens such as the rhizoctonia cerealis, the gaeumannomyces graminis, the fusarium graminearum, the fake fusarium graminearum and the bipolaris sorokinianum can be simultaneously detected; the PCR primer is high in sensitivity: 0.39ng of a pathogen DNA (deoxyribonucleic acid) sample can be detected: and the PCR primer is exact: compared with the conventional separation identifying and symptom identifying method, the PCR primer is higher in the accuracy of the detecting result. |
| priorityDate | 2012-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.