http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102787114-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b4711b7defc1422cdb7f86d2c95657bf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2012-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0270a074c426cfada5d667fafdf04b8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17ba349edd6f3b05946e9708200349d8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4573fe30940872ee983322cfc3830164 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_063156380a625bebd2c88d3364770c12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eafdafc0b6933cddb3c53920e9e2c068 |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102787114-B |
| titleOfInvention | Efficient directional seamless DNA (deoxyribonucleic acid) segment connecting method |
| abstract | The invention belongs to the technical field of gene engineering, in particular to a directional seamless DNA (deoxyribonucleic acid) segment connecting method. According to the method, 5 to 40 basic groups with completely complementary sequences are respectively arranged at the two ends of connecting primers and parts to be connected of DNA segments 2a and 3a to be connected; because the tm value of the primer is increased along with the increase of the GC content and the basic group number, and the active temperature interval of Taq DNA ligase for completing the connecting reaction is between 65 DEG C and 50 DEG C, so the number of the completely complementary basic groups between the connecting primer 1 and the single connecting segment needs to meet the requirement that the tm value between the connecting primer and the single connecting segment is between 50 DEG C and 65 DEG C; the segments are firstly subjected to phosphorylation, or the primer is subjected to phosphorylation, and then, PCR (polymerase chain reaction) amplification is carried out for ensuring the phosphoric acid group existence at the 5' end of the segments to be connected. The sequence position staggering cannot be generated in the connecting process, the serial connection oligomer is not generated, the self ring connection of carriers and segments is avoided, the operation freedom is high, the operation is simple, the period is short, and the price is low. |
| priorityDate | 2012-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.