| abstract |
The invention relates to a preparation method of a temperature sensitive type monolithic column with a chiral molecule recognition function, comprising the following steps of: on the basis of excellent performances of an organic polymer monolithic column, such as wide material sources, wide pH application range, introducing an cyclodextrin derivative which has specific separation capability on a chiral substance; and adding N-isopropyl acrylamide with the temperature sensitive characteristic into a reactant, wherein the environment temperature varies nearby LSCT of the N-isopropyl acrylamide, the molecular chain morphology of the N-isopropyl acrylamide varies, and a binding constant of a guest molecule and beta-cyclodextrin is mutated, so that adsorption and desorption of molecules are realized, and the temperature sensitive type monolithic column with the specific separation capability on the chiral substance is prepared. The monolithic column has an important application value in the field of chiral separation and analysis of complex samples. |