http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102747102-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70fbe08b7c540157200696115221f654 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56fcccfff0a5c5e2cc9fb21ee1b31519 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-873 |
filingDate | 2012-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4047297a8ec3c892e95dabfa66eb199b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4c4d1d753379960363fdf5ef9b1658ac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fea82842bda53e569902f219f8b2b66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5fb96d1db763724c9597126740705e79 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f912d986b820a345ff6a388d44943db http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e22a842a1df8338b3d9c83ba5a31dac5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28132c483cb5e89479dd5f506d91e25d |
publicationDate | 2014-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102747102-B |
titleOfInvention | HSA (Human Serum Albumin) mammary gland specific expression vector and reconstitution cell constructed by HSA mammary gland specific expression vector |
abstract | The invention discloses an HSA (Human Serum Albumin) mammary gland specific expression vector and a reconstitution cell constructed by the HSA mammary gland specific expression vector. The HAS mammary gland specific expression vector comprises a target gene HSA located at the downstream of a promoter with a 5' end connected with a BCP5 sequence and a 3' end connected with a BGHpolyA sequence, and a phiC31 integrase specific recognition site located at the upstream of the promoter. The 5' end of the target gene HSA is inserted with a bovine beta-casein gene and the 3' end of the target gene HSA is inserted with bovine growth hormone polyA, so that a 5' regulation and control region of the bovine beta-casein gene and the BGHpolyA can guide the target gene for specific high-efficiency expression in bovine mammary tissues. Bovine fetal fibroblast cells with the target genes HSA are taken as nuclear donor cells for nuclear transfer constitution of transgenic clone embryos, the transgenic clone embryos are obtained through SCNT (Somatic Cell Nuclear Transfer), and then, the embryos are transferred into uteruses of receptor cattles, so that milk cattles with HSA transgenic genes are hopefully produced. |
priorityDate | 2012-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 482.