| abstract |
The invention discloses a chiral pseudonucleoside compound, a preparation method and application thereof. The compound is shown in formula I. The raw materials for preparing pseudonucleoside compounds provided by the invention are obtained by catalyzing the hydrolysis of nitrogen heterocyclic diamide compounds by Rhodococcus erythropolis AJ270 microbial system. The amount of rhodococcus thalli used can be adjusted according to the amount of substrate. The reaction solvent is a common buffer solution with a pH value of 6.0-8.0, the temperature is 20-37° C., and the reaction time is 0.1-120 hours. The rhodococcus microbial catalytic system has the characteristics of fermentable culture and convenient storage. Using this biotransformation method to prepare chiral nitrogen heterocyclic diamides, monoamide carboxylic acids, and dicarboxylic acids has the advantages of simple operation, high reaction efficiency, mild reaction conditions, high enantioselectivity, easy separation of products, and high product purity. characteristics, and used for the synthesis of new pseudonucleosides. (Formula I) |