http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102732505-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5cedae1e4103c4e3719a5e9c94c2b510 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ca2d533777c3297826772586fc44212 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77920e74db6218cfc3699a3ac7d6ec0c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45b0c4b00a8cbe5c1e14d24670e5a2c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd4ede8af2b78c1db43324763c0161ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e7d7403c5b33986ecbf587bcc54b667 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1f5410942a1f60ea4304b2ee19fa336 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29d8391b42df15634a2325d2bb0bb80b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5d4e616611641af94e912fcae4b8b68 |
| publicationDate | 2014-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102732505-B |
| titleOfInvention | In vitro screening method of serum nucleic acid aptamers by multi-step concatenating selection |
| abstract | The invention discloses an in vitro screening method of serum nucleic acid aptamers by multi-step concatenating selection, belonging to the technical field of biomedical detection and analysis, characterized by eliminating individual difference by using a mixed serum as a screening target, conducting reverse screening by using a normal serum to remove nucleic acid sequences capable of combining with non-specific serum components from a library, then conducting screening by using the mixed serum to clone, sequence and separate nucleic acid aptamers, and selecting the nucleic acid aptamers having large application value by carrying out structural simulation analysis, singularity analysis, affinity analysis, and application value analysis on the nucleic acid aptamers. According to the invention, interference of large amounts of non-specific components in the serum is overcome, the method can directly use the serum as the target to screen out the nucleic acid aptamers having disease-specific components from the serum with high through-put, has the advantages of efficiency and economy, can lay the foundation for establishing analysis technology based on nucleic acid aptamers, systematically exploring the rules of the occurrence and development of diseases, and acquiring bioinformation related to clinic diagnosis and treatment, and has good application value. |
| priorityDate | 2012-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.