http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102732504-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_28f8a23ff177ec96b5d92557a09b1d7d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2011-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d866cb53e3e757bbac75fc98824e27c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_564d768d1c3f25d7798736fbeacb7bdb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8fc419d577cf7827f22d453b57456c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce238fa2daa292f481d2a71ca54d48fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4019d842c4fa88ddf4d991e1dcb293ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4710252a82a6b6c8b8a149debc1b2318 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c50f3d01e4509cb08d121fc69e707dac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d91f1c4f031a666c1b0100c090b701d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aff6826a0da97a904ccc953c3d348504 |
| publicationDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102732504-B |
| titleOfInvention | Method for extracting microorganism macrogenome from oil/gas pool environment |
| abstract | The invention provides a simple method for directly extracting microorganism macrogenome from an oil/gas pool environment sample, which comprises the following steps: an organic phase/water phase is extracted to obtain an microbe component in an oil sample/oil-water sample, vacuum filtering is carried out on the water phase enrichment microbes, cells are cracked by lysozyme, protease, sodium dodecyl sulfate (SDS) and the like, passes through the cetyl trimethyl ammonium bromide (CTAB) under high salt condition to remove organic macro-molecule components like protein and polysaccharide, extraction is carried out through nucleic acid and deposition is carried out, thereby the unbiased oil/gas pool microorganism macrogenome component can be obtained. The method of the invention has the advantages that the process is fast and simple, the cost is low, the whole extraction process can be completed in 10 hours; the obtained genome enables high purity, the ratio of OD260nm to OD280nm is between 1.7 and 2.0, the electrophoresis result detects that the integral genome possesses no degradation condition. The method of the invention aims at the oil/gas pool complex environments, and is suitable for extraction of other microbe genome in the soil and water body polluted by petroleum. |
| priorityDate | 2011-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.