http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102732484-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca74ed15eb9426695541087e53bd9e00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-095 |
| filingDate | 2012-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6458ab75002e83abe3b71f06b35fbbb9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4490a025cf49654c5eeb1695cc2abab8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5811d644675571ecc9b795a2fe0e3a01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f91bf32edf6c44a546505a87130c5371 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8258bbb744474c30f5641c9d7ee08c63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_caac9a5b42d8c58b8c0e72193eb7d979 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39880f247bc845da3cc6712c13121719 |
| publicationDate | 2015-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102732484-B |
| titleOfInvention | Method for establishing human nasopharyngeal carcinoma tumor stem cell line |
| abstract | The invention provides a method for establishing a human nasopharyngeal carcinoma tumor stem cell line. The method comprises the following steps: (1) obtaining human nasopharyngeal carcinoma cells in the single cell state; (2) adding the human nasopharyngeal carcinoma cells in the single cell state, obtained in the step (1), to a serum-free stem cell culture medium containing epidermal growth factors and basic fibroblast growth factors to obtain single cell suspension; (3) adjusting the concentration of the single cell suspension in the step (2) to 900-1100 single cells/ml; (4) inoculating 1ml of the single cell suspension obtained in the step (3) into single pores on a cell culture six-pore plate and then adding the equivoluminal stem cell culture medium for culture for 72-96 hours; and (5) collecting the cells obtained in the step (4), carrying out trypsinization till the single cell and carrying out subculture. The method provided by the invention is simple and convenient to operate; and the obtained nasopharyngeal carcinoma stem cell line has obvious stem cell characteristics. |
| priorityDate | 2012-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.