http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102721810-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2c27f4a786895417d03d0b1045587100 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2012-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9da8bec8bb7d2625344b86204749dff4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0892fa9b4a9e33d0a21d5b434e75287 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10db74d479b88aff61a57fc136b46b06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5016c79553f0b58f0b12245092b5d788 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_680d8da3142c34b2460a05b3edcf490a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d07ef00f130c3e747e4904204baffd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e1bbc7d1d49d182d0a6b0ad167c3ec9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c979a709fc2d03b8da8a26459518978 |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102721810-B |
| titleOfInvention | Liquid-phase blocking ELISA kit for discriminating classic PRRS and HPRRS |
| abstract | The present invention relates to a liquid-phase blocking ELISA kit for discriminating classic PRRS (porcine reproductive and respiratory syndrome) and HPRRS (porcine reproductive and respiratory syndrome virus). A method using the kit is characterized by comprising the steps of: diluting a serum to be tested with the sample dilution solution by two times, adding 100 mul into each well of an ELISA plate, incubating at 37 DEG C for 1h, and washing with the washing liquid for 5 times for 2 min each time; then adding 100 mul of a binding monoclonal antibody into each well, incubating at 37 DEG C for 1h, and washing with the washing liquid for 5 times with 2 min each time; then adding a goat anti-mouse IgG-HRP conjugate, reacting at 37 DEG C for 1h, and washing with the washing liquid for 5 times for 2 min each time; then adding a chromogenic substrate solution, mixing a substrate A with a substrate B by 1:1, adding 100 mul into each well of the ELISA , and carrying out chromogenic reaction away from light for 15 min, adding 50 mul of a terminated liquid and measuring an OD490 value. The method using the kit is mature, has strong repetitivity, can effectively discriminate the classic PRRS and HPRRS antibody, and can be completed by general researchers. |
| priorityDate | 2012-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.