http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102719465-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b4711b7defc1422cdb7f86d2c95657bf |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2011-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3595a110b5ba6678aea2d90da15ae393 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44ddbaa62a29a01a1c000442907d0992 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f71d4f622a39271e4cc50759ceb9426f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83ece33c4fc880b988f6a3a9a39931f2 |
| publicationDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102719465-B |
| titleOfInvention | Inducible tissue-specific expression vector and purpose thereof |
| abstract | The invention belongs to the field of biological technology, relating to an inducible tissue-specific expression vector and a purpose thereof. The inducible tissue-specific expression vector mainly comprises the following structures of: a promoter EF1 alpha, an EGFP fluorescent marker gene, a BGHpoly A tailing transcription termination sequence with two ends linked with same-direction Lox sequences, and an intron sequence with internal enzyme sites introduced. The vector is pEFGLPAL-MIR using a general promoter EF1alpha to drive gene expression; the EGFP fluorescent marker gene is used for indication of the gene expression; two BDHpoly A tailing transcription termination signals linked with same-direction LoxP71 and LoxP66 are inducible by enzyme Cre. The termination signals are followed by an intron sequence, with an miRNA expression precursor is inserted into the intron. The micro RNA expression vector can successfully express specific micro RNA and the micro RNA expression can be determined by various ways. The expression vector is capable of expressing uneasily-silenced micro RNA in an ES cell, so that can be successfully applied in the preparation of transgenic mice. |
| priorityDate | 2011-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.